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spheroid-qualified phhs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher spheroid-qualified phhs
    Spheroid Qualified Phhs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phh+spheroids/spheroid+qualified+phhs/pm37235733-115-5-9
    Average 90 stars, based on 1 article reviews
    spheroid-qualified phhs - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: 3D co-cultures of primary human hepatocytes and Kupffer-like cells to address innate immune responses to rAAV.
    Article Snippet: Cells were 503 resuspended at 50 X 106 cell/mL and monocyte isolation was performed employing the 504 EasySep human monocyte isolation kit (STEMCELL Technologies), following the 505 manufacturer’s instructions. .. Cell concentration was determined by AR TIC LE IN PR ES S 30 519 digesting a sample of PHH spheroids with 0.05% Trypsin/EDTA (Thermo Fisher 520 Scientific) and the resulting single-cell suspension was counted using a Fuchs521 Rosenthal chamber by the trypan blue exclusion method. ..

    Single Cell:

    Article Title: 3D co-cultures of primary human hepatocytes and Kupffer-like cells to address innate immune responses to rAAV.
    Article Snippet: Cells were 503 resuspended at 50 X 106 cell/mL and monocyte isolation was performed employing the 504 EasySep human monocyte isolation kit (STEMCELL Technologies), following the 505 manufacturer’s instructions. .. Cell concentration was determined by AR TIC LE IN PR ES S 30 519 digesting a sample of PHH spheroids with 0.05% Trypsin/EDTA (Thermo Fisher 520 Scientific) and the resulting single-cell suspension was counted using a Fuchs521 Rosenthal chamber by the trypan blue exclusion method. ..

    Suspension:

    Article Title: 3D co-cultures of primary human hepatocytes and Kupffer-like cells to address innate immune responses to rAAV.
    Article Snippet: Cells were 503 resuspended at 50 X 106 cell/mL and monocyte isolation was performed employing the 504 EasySep human monocyte isolation kit (STEMCELL Technologies), following the 505 manufacturer’s instructions. .. Cell concentration was determined by AR TIC LE IN PR ES S 30 519 digesting a sample of PHH spheroids with 0.05% Trypsin/EDTA (Thermo Fisher 520 Scientific) and the resulting single-cell suspension was counted using a Fuchs521 Rosenthal chamber by the trypan blue exclusion method. ..

    other:

    Article Title: 3D co-cultures of primary human hepatocytes and Kupffer-like cells to address innate immune responses to rAAV
    Article Snippet: PHH 3D culture viability was assessed by fluorescence microscopy using 1 μg/mL propidium iodide (PI; Merck) to label dead cells.



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    Image Search Results


    Assigned DILI category (Chen et al. <xref ref-type= 2016 or literature alignment), plasma C max.tot values, f u values, MEC and AC 50 values for hLiMT’s and HepaRG spheroids in the high-content screening (HCS) and cellular ATP endpoints" width="100%" height="100%">

    Journal: Archives of Toxicology

    Article Title: The evolution of strategies to minimise the risk of human drug-induced liver injury (DILI) in drug discovery and development

    doi: 10.1007/s00204-020-02763-w

    Figure Lengend Snippet: Assigned DILI category (Chen et al. 2016 or literature alignment), plasma C max.tot values, f u values, MEC and AC 50 values for hLiMT’s and HepaRG spheroids in the high-content screening (HCS) and cellular ATP endpoints

    Article Snippet: AstraZeneca evaluated the use of a human liver-chip model versus a PHH liver spheroid model for acetaminophen (APAP) and fialuridine (FIAU).

    Techniques: Clinical Proteomics, High Content Screening, Tandem Mass Spectroscopy